In collaboration with the WI DNR, GNFH is planning to restore Snuffbox populations in the Wolf River basin. Last fall, WI DNR biologists spent some chilly dive days aggregating males and females to ensure that brooding females could be found this spring. The first tiny babies (~150 ?m long) are expected soon. Soon these will go out into the Mobile Aquatic Rearing System at Blackhawk, the U.S. Army Corps Park, to eat the natural food in Mississippi river water (bacteria and algae) and grow large enough to overwinter well at the hatchery for release in 2023.
Snuffbox is a particularly favorite species as it’s the only widespread member of their genus, all of whom catch their host fish. This unexpected behavior begets novel characteristics (‘toothed’ shell edges, the capacity to sit open, exposing their body cavity to the environment) making these species fascinating to observe. How these behaviors and morphologies, or physical characteristics in biology jargon, have developed and been maintained is fascinating to consider. We look forward to sharing more information about this species and others reared at the hatchery as our buildings open to the public and events develop in the coming years.
The group of broodstock Snuffbox, freshly tagged and ready to be returned to the river.
Snuffbox use log perch as hosts.
The Hine’s Emerald Dragonfly eggs that GNFH has been housing since the end of December have all hatched. The eggs were held at 39-41 degrees over the winter and, in early April as the outside temperatures began to rise, we began warming the eggs. By end of April they had warmed to 60 degrees, and almost 1000 larvae had hatched. Egg cups were checked every day, and the day’s newly hatched larvae were placed in small specimen cups of pond water with a few zooplankton items as food.
These juveniles were then distributed into small rearing cups either individually or in groups of two or three individuals per cup. These cups, kept in groups by maternal line, are being housed at 60 degrees and fed three times a week by pipetting pond water with concentrated zooplankton filtered out of the pond water that comes through the mussel building. Food water is checked for larger pests that will view the dragonflies themselves as food (for example chironomids, some species are predatory). The remaining copepods, ostracods, cladocerans, and an early April bloom of rotifers- which appear to be a preferred food for new instar larvae- are available for the little dragonflies to hunt as prey.
After the larvae grow past their first few instars in these protected cups, they’ll move up to the next stage- small pvc and mesh cages submerged in a larger volume of water, with more food available. Individuals have to have grown large enough to stay enclosed in mesh with 500-micron openings—this size is large enough to allow water flow to bring in oxygen and new food items in without clogging too quickly the our productive pond water with algae and detritus. The photos below (bottom row) show an early and later instar juvenile on this screen as a check to see if this mesh size will contain the individual (answers: not yet, and yeah, definitely!).
By: Beth Glidewell
A three week old and 5 month old dragonfly larva on the same size mesh screen. Photos: Beth Glidewell/USFWS.
After a missed year of spawning Walleye on Pool 9 in 2020 due to the pandemic, we were back in action this spring. A strange spring it was weather wise for us in Western Wisconsin. We put our nets in on April 5 and started spawning fish on April 6. An unseasonably warm spell following a cold snap had temperatures rise 10 degrees from 44 F to 54 F in only three days, peaking at 58 F a couple days later which blew right through the typical spawning temperatures (around 45-50 F). A cold snap brought temps back down into the mid 40’s the following week. The fish seemed to be confused as well.
We never saw the typical “bell curve” of spawning activity slowing rising to a peak before slowly falling off. Numbers of ‘ripe’ fish were inconsistent until falling to near zero by April 22 when we pulled our nets. We likely missed a few of the early spawners over Easter weekend with the significant warm up, but we were able to send millions of eggs to our partners, stock our ponds for production, and return hundreds of thousands of eggs and fry back to the river.
By: Nick Bloomfield
Zach mixing milt with freshly collected eggs
Nick and Chelsea collecting newly hatched fry for stocking. Photos: Beth Glidewell/USFWS.
If you’ve been reading the newsletter for a while, you’re aware of the cycle of the year. Winter is for managing data and making plans, while spring is for preparation, summer is busy with hands on work with the mussels on station or in the river, then fall is bringing in the animals we’ve raised and settling them in for the winter. So much of that has stayed the same with the ongoing pandemic and but the planning has gotten more interesting. Winter is still important for being prepared for the year. We start by reviewing what was successful last year before deciding what our priorities are for the next. Some of this work includes assessing the status of cages, preparing dive gear for another season, and coordinating plans with our partners. We’ve had discussions with our various State and Federal partners, as well as the staff at the National Mississippi River Museum and Aquarium in Dubuque, about plans for the coming season. We’re still providing extensive care for several species of host fish and the many sub-adult mussels that are overwintering in the mussel building even as we finish construction on some new systems in the mussel building.
By: Megan Bradley
The new rearing pan system in the mussel building, with hoses delivering winter-temperature pond water and nutrients to the juvenile mussels that call these pans home from October-November until approximately June, when they’ll head to an outdoor culture system for the growing season. Photo: Megan Bradley/USFWS
Orey displaying an adult brook trout that was part of the 2017 egg collection effort
The newest additions to Genoa National Fish Hatchery arrived on station in January. About 50,000 fertilized Brook Trout eggs were sent from our sister hatchery at Iron River. Hatching began almost immediately upon arrival in mid-January and all the fry were hatched out by the end of January. Staff have been busy caring for the eggs and fry ever since. These fish will live in the safety of the quarantine building until they have passed multiple fish health inspections. The treatment system in place in the Quarantine Building will keep the rest of the hatchery protected from any pathogens until we can ensure that there are none, at which point we can move them to a bigger rearing space. Once these fish are big enough to ensure a high survival rate in the wild, they will be stocked into the Lake Superior basin to help boost populations of Coaster Brook Trout.
The story behind the origin of these new arrivals begins in October of 2017. Eggs were collected from remnant populations of Coaster Brook Trout from the Isle Royal area and raised at Genoa NFH. This process was repeated the following year, and another batch grew up in the hallowed raceways of Genoa NFH. These fish weren’t destined for the wild, however. These two lots were destined for the FWS broodstock program and were sent back to Iron River NFH. Fast forward to 2021 and holding two distinct lots of broodstock that were becoming sexually mature, staff at Iron river NFH were able to cross them with minimal likelihood of relatedness between parents. These little guys will grow up in the same raceways their parents did, but they are destined to ultimately help grow the population along the shores of Lake Superior so Americans can enjoy them for generations to come.
By: Nick Bloomfield
several hundred Hine’s Emerald Dragonfly eggs viewed through a dissection scope,
A few weeks before the New Year, a batch of Hine’s Emerald Dragonfly eggs arrived on station at Genoa NFH. This new cohort, approximately 1000 eggs collected from 6 females, are part of a collaborative captive rearing effort that Genoa has participated in since 2015, along with partners from the University of South Dakota, Illinois DNR, Forest Preserve District of DuPage County, IL, and the USFWS Chicago Field Office.
This season’s eggs are currently being kept in clean well water at a stable 4C, a temperature that delays hatching until conditions are more favorable in the spring. In late March- early April we’ll start warming the eggs and check daily for hatching juveniles. Until then, eggs are checked at least weekly, being monitored for early hatchlings and problems such as fungal growth.
So far there have only been a few early hatches. These new larvae are acclimated to pond water and slowly warmed to room temperature, 15-16C. The warmed pond water contains a variety of zooplankton that the dragonfly larvae- tiny but fierce predators- will utilize as food. The larvae will remain in individual culture cups for several months, protected from pests and growing larger size. When outside pond water has warmed to similar culture temperatures -May to June- the larvae will be placed in small mesh enclosures in rearing tanks in GNFH’s ‘dragonfly trailer’, and later into larger mesh cages in ponds at the hatchery for the 2021 growing season.
We also hope to culture Devil’s Crayfish on station this year. 2020 saw the addition of this species to the hatchery, as a culture trial to check the feasibility of culturing crayfish at GNFH. The animals on station did well, growing and successfully molting, so we hope this effort will continue in the coming years. Devils Crayfish are being studied and cultured in tandem with Hine’s Emerald Dragonflies as it has been observed that the crayfish make burrows and chimneys in wetland sediments that the Hine’s Emerald larvae utilize as habitat.
By: Beth Glidewell
a newly hatched larvae,
An exciting offshoot of the Genoa National Fish Hatchery (GNFH) role in the Maumee River (OH) Lake Sturgeon restoration work is to be able to contribute fish to Michigan’s Lake Huron basin restoration program in cooperation with the Michigan DNR. Lake sturgeon are broadcast spawners, meaning they literally release hundreds of thousands of eggs per female in order to achieve reproductive success. This is due to the many predators that wait for a meal of sturgeon eggs every spring. Due to this large number of eggs that are released, spawning crews generally take more eggs than needed from specific females in order to maintain strain specific restoration programs.
This has specifically paid benefits on the western shore of Lake Huron where the GNFH joins forces with many partners such as the Michigan DNR, the Ontario Ministry of Natural Resources and Forestry, Alpena Fish and Wildlife Conservation Office and others to collect eggs annually from fish captured in the Upper St. Clair River. The fish are held and eggs collected at a local commercial fishery that graciously allows us to use their holding facilities to restore this species to the Great Lakes watershed. In the years 2017, 2018 and 2019 a total of 2,059 Lake Sturgeon fingerlings were stocked into tributaries which flow into the Saginaw Bay area in Lake Huron.
These efforts are already producing results with fish being captured and released in waters devoid of sturgeon for the last 100 years. This after only a stocking effort of 3 year classes and a little over 2,000 fingerlings! We hope to contribute many more year classes and their important genetic contribution in order to ensure both adequate numbers of fish are able to sustain the new population, and significant amount of genetic variation is available for the population to remain viable. However, it is heartening that although such a small number of fish were released, they are already being seen and documented in habitat that has been devoid of the species. We find hope that this is a sure sign that our efforts and the efforts of our partners have a hand in the return of this magnificent species.
By Doug Aloisi
An angler holds lake sturgeon caught in the Saginaw River. Fish was released
Ontario Ministry of Natural Resources and Forestry’s Chatsworth and Normandale Fish Culture Stations staff(left) Riley Hotrum and (right) Steffi Krauseholding an adult lake trout. Riley is a technician with the Upper Great Lakes Management Unit, and Steffi isChatsworth Fish Culture Station’s Operations Coordinator. Photos provided byMatthew Brailey, Manager, Chatsworth Fish Culture Station and Normandale Fish Culture Station,OMNRF.
Genoa National Fish Hatchery received lake trout eggs from our partners in Ontario, Canada (Chatsworth and Normandale Fish Culture Stations). Staff from these facilities collected wild adult lake trout from Big Sound, Lake Huron. Eggs were incubated at their facilities until they were eyed and shipped by mail to Genoa National Fish Hatchery. Upon receiving eyed eggs, they were disinfected with iodine and incubated in heath trays at water temperatures between 7-8 °C in an insulated recirculating system in one of Genoa’s regional isolation buildings. Once these eggs hatch the fish will remain on station for a year and a half until they clear three fish health inspections by the La Crosse Fish Health Center (La Crosse, WI). Once all testing comes back clear, the fish will be transferred to their forever home at one of our broodstock facilities (Pendills and Sullivan Creek National Fish Hatcheries, MI) where eventually they will be used to produce eggs for the lake trout hatcheries to grow into yearlings for restoration stocking efforts in Lake Huron.
By: Orey Eckes
Angela stocking fry into a hatchery pond.
Angela Baran Dagendesh, Genoa (WI) National Fish Hatchery’s assistant project leader, has accepted a new position in the Washington office within our Refuge Branch. Angela will be a Facility Operations Specialist, assisting in the Refuge Quarters management and real property management. Angela came to Genoa way back in 2010, and is leaving quite a legacy and large shoes to fill. With her passion for the resource, she applied for and received a Cooperative Conservation grant for the hatchery that propelled us to begin rearing the Hines Emerald Dragonfly, the first federally endangered aquatic insect to be cultured in the federal hatchery system. This past year, she developed protocols for bringing Devils Crayfish on to the station, a species that assists the Hines Emerald Dragonfly to overwinter safely in their burrows. For her work with the dragonfly, Angela received the Midwest Region’s Endangered Species Recovery Champion award in 2016. She also was instrumental in running the station’s production program, with many new sturgeon restoration efforts beginning and thriving during her tenure. We will miss her competence and passion for the Resource in the years ahead. Best of luck Angela! Thank you for a great decade! By Doug Aloisi
Newly transformed terrestrial stage Hines Emerald Dragonfly. USFWS photos.
As production season winds down, things start to slow down a bit at the hatchery. However, that doesn’t mean we don’t have fish on station. We hold on to several species for different reasons throughout the winter. We currently have four of our ponds set up to overwinter fish.
One of these ponds is dedicated to finish growing out the Rainbow Trout hatched last winter. These will be grown as much as possible until the end of April when they will be stocked out as catchable sized trout. Another pond is dedicated to keeping our “fishing day” fish, mainly Rainbow Trout with some fun surprises sprinkled in. There are some absolute monsters in there, partially due to the fishing day cancellations in 2020. The next fishing event will be epic, whenever that might be!
Another pond is dedicated to all the broodstock sportfish on station, including Yellow Perch, Bluegill, Largemouth Bass, Smallmouth Bass, and Black Crappie. They will be divided into their own ponds come spring. Finally, a pond is used by some of this years’ Walleye and Smallmouth Bass crops. These are destined to become host fish for the mussel program in 2021. More current and future mussel host fish can be found inside the buildings. Channel Catfish, Freshwater Drum, Walleye, Golden Shiner, and Flathead Catfish are all waiting their turn to carry around some baby mussels. Never a dull moment!
By: Nick Bloomfield
One of the broodstock ponds with the 20-21 season’s first dusting of snow. USFWS photo
Feeding the Rainbows- an automatic feeder distributes feed to hungry Rainbow Trout. USFWS photo.